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Definition of CRISPR
CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats. In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated system) genes are located next to CRISPR sequences. A simple version of the CRISPR/Cas system, CRISPR/Cas9, has been modified to edit genomes. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added.
Q & A About CRISPR including a video by Feng Zhang, hosted by the Broad Institute.
General Information on CRISPR
Researchers the world over are fast adopting CRISPR-Cas9 to tinker with the genomes of humans, viruses, bacteria, animals and plants. Nature brings together research, reporting and expert opinion to keep you abreast of the frontiers of gene editing.
The Heroes of CRISPR
Cell, Volume 164, Issues 1–2, 14 January 2016, Pages 18-28.
Stanford scientists combine CRISPR and DNA barcoding to track cancer growth
April 3, 2018: Cancer research that once involved years of painstaking work can now happen in months with a novel technique for systematically studying cancer-related genes. The results reveal how combinations of mutations influence tumor growth.
A global observatory for gene editing
Nature 555, 435-437 (2018).
Sheila Jasanoff and J. Benjamin Hurlbut call for an international network of scholars and organizations to support a new kind of conversation.
CRISPR: Social Media & News Links
Books at the DePauw Libraries
A Crack in Creation by
Call Number: Prevo Science Library General Collection QH442 .D68 2017
Publication Date: 2017-06-13
Two Berkeley scientists explore the potential of a revolutionary genetics technology capable of easily and affordably manipulating DNA in human embryos to prevent specific diseases, addressing key concerns about related ethical and societal repercussions.
Modern Prometheus by
Call Number: Prevo Science Library General Collection QH442 .K69 2016
Publication Date: 2016-10-18
Would you change your genes if you could? As we confront the 'industrial revolution of the genome', the recent discoveries of Crispr-Cas9 technologies are offering, for the first time, cheap and effective methods for editing the human genome. This opens up startling new opportunities as well as significant ethical uncertainty. Tracing events across a fifty-year period, from the first gene splicing techniques to the present day, this is the story of gene editing - the science, the impact and the potential. Kozubek weaves together the fascinating stories of many of the scientists involved in the development of gene editing technology.
Nature CRISPR Video: from October 31, 2017