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Genome Editing: the brave new world of CRISPR: Home

This guide has been created to support the CRISPR events at DePauw April 2-6, 2018.

Definition of CRISPR

CRISPR is an abbreviation of Clustered Regularly Interspaced Short Palindromic Repeats.  In a palindromic repeat, the sequence of nucleotides is the same in both directions. Each repetition is followed by short segments of spacer DNA from previous exposures to foreign DNA (e.g., a virus or plasmid). Small clusters of cas (CRISPR-associated system) genes are located next to CRISPR sequences. A simple version of the CRISPR/Cas system, CRISPR/Cas9, has been modified to edit genomes. By delivering the Cas9 nuclease complexed with a synthetic guide RNA (gRNA) into a cell, the cell's genome can be cut at a desired location, allowing existing genes to be removed and/or new ones added.

 

Q & A About CRISPR including a video by Feng Zhang, hosted by the Broad Institute.

General Information on CRISPR

CRISPR Research Articles

CRISPR TED talk

Books at the DePauw Libraries

Nature CRISPR Video: from October 31, 2017

Librarian

Caroline Gilson's picture
Caroline Gilson
Contact:
x4404
cgilson@depauw.edu
Prevo Science Library, Julian 174